sodF [2019-07-03 08:43:13]

superoxide dismutase

locus: BSU19330

pI: 6.17

mw: 33.32 kDa

protein length: 281 aa

gene length: 846 bp

function: detoxification of oxygen radicals

product: superoxide dismutase

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]

Gene

Coordinates: 2,104,056 → 2,104,901

The protein

Catalyzed reaction/ biological activity

• 2 superoxide + 2 H⁺ = O₂ + H₂O₂ (according to Swiss-Prot)

Protein family

• terpene cyclase/mutase family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|6153A07B961017052E1072944B59F510D80CEC0F|SodA]

[SW|Cofactors]

• contains an iron-sulfur cluster

Structure

• [PDB|3EVK] (aa 80 ... 280, from Pyrobaculum aerophilum, 54% identity)

Expression and Regulation

Operons

• genes: [gene|24EB23F594F2B0EEA2E055857BA14FD9E361E0FD|sqhC]-[gene|E0645DD4A7A871457300139A7E5986EF11C07387|sodF]
  description: [Pubmed|18436644]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|15699190,18436644], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

  regulation

  • expressed during sporulation in the mother cell [Pubmed|18436644]

Biological materials

Mutant

• BKE19330 (Δ[gene|E0645DD4A7A871457300139A7E5986EF11C07387|sodF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE19330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATTGAACATCTCCGCTTGAT, downstream forward: _UP4_TAAAAAAAGCCCCCTTTCCT
• BKK19330 (Δ[gene|E0645DD4A7A871457300139A7E5986EF11C07387|sodF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK19330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATTGAACATCTCCGCTTGAT, downstream forward: _UP4_TAAAAAAAGCCCCCTTTCCT

References

• 18436644